The Novusyn Research semaglutide reconstitution calculator is a laboratory math tool for investigators preparing lyophilized semaglutide research vials. Enter the vial mass (mg), the bacteriostatic water volume added (mL), and a target mass per draw (mg); the tool returns the resulting concentration in mg/mL, the draw volume in mL, and the equivalent U-100, U-50, or U-40 insulin-syringe units. All outputs are theoretical calculations for in-vitro research planning. The tool is not medical instruction, not a dosing schedule, and not a substitute for the judgment of a licensed clinician. Site access is restricted to users 21+ per our compliance policy and the broader editorial standards.
Semaglutide is supplied as a lyophilized powder in research vials commonly sized 2 mg, 3 mg, 5 mg, and 10 mg. Bacteriostatic water (0.9% benzyl alcohol) is the standard reconstitution solvent. The chosen BAC water volume determines the final concentration and therefore the draw volume required for any given target mass. Higher concentrations reduce draw volume; lower concentrations improve resolution on a U-100 barrel. Researchers should select a concentration that permits accurate volumetric measurement at the resolution of the syringe in use.
Reference concentrations. 5 mg vial + 1.0 mL BAC water = 5 mg/mL. 5 mg vial + 2.0 mL BAC water = 2.5 mg/mL. 10 mg vial + 1.0 mL BAC water = 10 mg/mL. 10 mg vial + 2.0 mL BAC water = 5 mg/mL. 2 mg vial + 1.0 mL BAC water = 2 mg/mL.
U-100 conversion at 5 mg/mL. 0.25 mg = 5 units. 0.50 mg = 10 units. 1.00 mg = 20 units. 1.70 mg = 34 units. 2.40 mg = 48 units. At 2.5 mg/mL: 0.25 mg = 10 units, 0.50 mg = 20 units, 1.00 mg = 40 units. These values are direct unit conversions of the underlying mg/mL math and assume an ideal solution.
Reconstitution checklist for research materials. Wipe the vial septum with 70% isopropyl alcohol and allow to dry. Draw the calculated BAC water volume into a sterile syringe. Dispense the BAC water slowly down the inner vial wall to minimize foaming and shear. Gently swirl the vial; do not shake or vortex peptide solutions. Allow 2–3 minutes for complete dissolution; the solution should be clear and colorless. Inspect for particulate matter or cloudiness and discard if either is present. Store reconstituted solution at 2–8°C and treat per documented stability data. Label the vial with batch number, reconstitution date, concentration, and expiration. See the full long-form Peptide Reconstitution Guide and the related How to Read a Certificate of Analysis.
Syringe selection. U-100 insulin syringes (1 mL capacity, 100-unit markings) provide the finest granularity for sub-milligram research math. U-50 and U-40 scales are also supported by the tool. Researchers should validate that the chosen concentration yields a draw volume measurable at the resolution of the syringe in use. For general (non-semaglutide) math, use the broader Peptide Reconstitution Calculator; for compound background, see the Semaglutide reference and the Tirzepatide comparison file. Ongoing trial coverage is collected in the GLP-1 trial tracker, regulatory developments in the FDA Peptide Regulatory Status brief, and daily updates in News. Definitions of unfamiliar terms are in the glossary.